UNIT 7.7 Analysis of DNA Content and BrdU Incorporation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Darzynkiewicz, Z. and Juan, G. 2001. Analysis of DNA Content and BrdU Incorporation. Current Protocols in Cytometry. 2:7.7:7.7.1–7.7.9.
- Published Online: 1 MAY 2001
- Published Print: OCT 1997
During the past two decades, the techniques of analysis of cell cycle kinetics based upon the flow cytometric detection of BrdU incorporation have replaced the cumbersome autoradiographic methodology utilizing tritiated thymidine. This unit distills the knowledge gained over this time into concise and easy to follow protocols for DNA content analysis following thermal denaturation or acid denaturation, as well as a modified method that combines thermal denaturation with washless immunocytochemical detection of BrdU.