Unit

UNIT 7.7 Analysis of DNA Content and BrdU Incorporation

  1. Zbigniew Darzynkiewicz,
  2. Gloria Juan

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0707s02

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Darzynkiewicz, Z. and Juan, G. 2001. Analysis of DNA Content and BrdU Incorporation. Current Protocols in Cytometry. 2:7.7:7.7.1–7.7.9.

Author Information

  1. New York Medical College, Valhalla, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1997

Abstract

During the past two decades, the techniques of analysis of cell cycle kinetics based upon the flow cytometric detection of BrdU incorporation have replaced the cumbersome autoradiographic methodology utilizing tritiated thymidine. This unit distills the knowledge gained over this time into concise and easy to follow protocols for DNA content analysis following thermal denaturation or acid denaturation, as well as a modified method that combines thermal denaturation with washless immunocytochemical detection of BrdU.