UNIT 7.11 Assessment of Viability, Immunofluorescence, and DNA Content

  1. Ingrid Schmid

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0711s10

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Schmid, I. 2001. Assessment of Viability, Immunofluorescence, and DNA Content. Current Protocols in Cytometry. 10:7.11:7.11.1–7.11.19.

Author Information

  1. UCLA School of Medicine, Los Angeles, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999


This unit addresses the difficult and important area of differentiation between viable and nonviable cells, a question of particular interest for DNA studies. The basic protocol describes the determination of DNA content using pyronin Y combined with dead cell discrimination using 7-AAD. Alternate protocols present methods for DNA content and viability assessment and simultaneous surface or intracellular antigen expression.