UNIT 7.11 Assessment of Viability, Immunofluorescence, and DNA Content
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Schmid, I. 2001. Assessment of Viability, Immunofluorescence, and DNA Content. Current Protocols in Cytometry. 10:7.11:7.11.1–7.11.19.
- Published Online: 1 MAY 2001
- Published Print: OCT 1999
This unit addresses the difficult and important area of differentiation between viable and nonviable cells, a question of particular interest for DNA studies. The basic protocol describes the determination of DNA content using pyronin Y combined with dead cell discrimination using 7-AAD. Alternate protocols present methods for DNA content and viability assessment and simultaneous surface or intracellular antigen expression.