Unit

UNIT 7.17 Analysis of Viral Infection and Viral and Cellular DNA and Proteins by Flow Cytometry

  1. John M. Lehman,
  2. Thomas D. Friedrich,
  3. Judith Laffin

Published Online: 1 AUG 2001

DOI: 10.1002/0471142956.cy0717s17

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Lehman, J. M., Friedrich, T. D. and Laffin, J. 2001. Analysis of Viral Infection and Viral and Cellular DNA and Proteins by Flow Cytometry. Current Protocols in Cytometry. 17:7.17:7.17.1–7.17.9.

Author Information

  1. Albany Medical College, Albany, New York

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: JUL 2001

Abstract

Viruses are obligate intracellular parasites that require the host cell replication, transcription, and translation machinery for reproduction. Each viral group provides a unique series of viral-cellular interactions. Studies have provided insight not only into viral replication and control of host functions, but also into cellular functions such as eukaryotic replication, transcription, and translation as well as the regulation of these events. This unit presents a protocol for flow cytometric monitoring of viral infection and quantitating viral-cellular events. The availability of monoclonal and/or polyclonal antibodies directed to both viral and cellular proteins offers the ability to assay a specific molecule in the intact fixed cell and the opportunity to correlate viral events with cellular processes such as progression through the cell cycle.