Unit

UNIT 7.22 Analysis of Tissue Imprints by Scanning Laser Cytometry

  1. Reinhard Bollmann1,
  2. Gábor Méhes2

Published Online: 1 NOV 2004

DOI: 10.1002/0471142956.cy0722s26

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Bollmann, R. and Méhes, G. 2004. Analysis of Tissue Imprints by Scanning Laser Cytometry. Current Protocols in Cytometry. 26:7.22:7.22.1–7.22.6.

Author Information

  1. 1

    Institute of Pathology Bonn-Duisdorf, Bonn, Germany

  2. 2

    University of Pécs, Pécs, Hungary

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2003

Abstract

The analysis of DNA content and simultaneous surface or intracellular antigen expression is described for laser scanning cytometry. Measurements can be performed on microscope slides prepared immediately after dissection of native surgical specimens. These protocols will be useful for both diagnostic pathology and cell biology laboratories.

Keywords:

  • Ploidy;
  • DNA;
  • immunofluorescence;
  • laser scanning cytometry;
  • breast cancer