Unit

UNIT 7.23 Cell Cycle Analysis of Budding Yeast Using SYTOX Green

  1. Steven B. Haase

Published Online: 1 NOV 2004

DOI: 10.1002/0471142956.cy0723s26

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Haase, S. B. 2004. Cell Cycle Analysis of Budding Yeast Using SYTOX Green. Current Protocols in Cytometry. 26:7.23:7.23.1–7.23.4.

Author Information

  1. Duke University, Durham, North Carolina

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2003

Abstract

Flow cytometric determination of DNA content is a standard tool for cell cycle analysis in the budding yeast, Saccharomyces cerevisiae. Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses. SYTOX-stained cells exhibit improved coefficients of variation (CVs), a better correlation between DNA content and fluorescence, and decreased sensitivity to variances in experimental conditions when compared with PI-stained cells. The SYTOX Green protocol provides researchers with a more accurate and reliable alternative to standard PI-based protocols for the analysis of the budding yeast cell cycle.

Keywords:

  • cell cycle;
  • SYTOX Green;
  • propidium iodide;
  • flow cytometry