UNIT 7.25 DRAQ5 Labeling of Nuclear DNA in Live and Fixed Cells
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Smith, P. J., Wiltshire, M. and Errington, R. J. 2004. DRAQ5 Labeling of Nuclear DNA in Live and Fixed Cells. Current Protocols in Cytometry. 28:7.25:7.25.1–7.25.11.
- Published Online: 1 MAY 2004
- Published Print: APR 2004
This unit describes the use of a novel DNA-detecting far-red-fluorescing dye, DRAQ5, a modified anthraquinone, which has a unique combination of properties exploitable by cytometry. These include a high capacity to permeate the cell membrane, a high DNA binding affinity and selectivity, a fluorescence emission spectrum beyond that of fluorescein, phycoerythrin, Texas Red, Cy3, and EGFP, and excitation characteristics separate from those of propidium iodide. In this unit, methods are presented for preparation and analysis of both live and fixed cells stained with DRAQ5. While the focus is on flow cytometric assays, typical imaging applications are also indicated because the staining protocols share the same essential features.
- DNA staining