Unit

UNIT 7.35 Assessment of Histone Acetylation Levels in Relation to Cell Cycle Phase

  1. Simona Ronzoni1,
  2. Mario Faretta1,
  3. Marco Ballarini1,
  4. PierGiuseppe Pelicci1,
  5. Saverio Minucci1,2

Published Online: 1 OCT 2008

DOI: 10.1002/0471142956.cy0735s46

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Ronzoni, S., Faretta, M., Ballarini, M., Pelicci, P. and Minucci, S. 2008. Assessment of Histone Acetylation Levels in Relation to Cell Cycle Phase. Current Protocols in Cytometry. 46:7.35:7.35.1–7.35.8.

Author Information

  1. 1

    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy

  2. 2

    Department of Biomolecular Sciences and Biotechnology, University of Milan, Milan, Italy

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008

Abstract

Histone acetylation affects chromatin structural organization, thus regulating gene expression and DNA-related cellular events. Levels of histone acetylation are tightly modulated in normal cells and frequently altered in tumors. Consequently, histone deacetylase inhibitors are currently being tested in clinical trials as anticancer drugs. Presented here is a protocol for measuring the degree of cellular histone tail acetylation, alone or in combination with DNA content to simultaneously evaluate cell ploidy and/or cell cycle progression. The procedure can also be employed to stain peripheral blood samples in order to assess mean histone acetylation levels in patients treated with histone deacetylase inhibitors. Curr. Protocol. Cytom. 46:7.35.1-7.35.8. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • flow cytometry;
  • histone;
  • acetylation;
  • histone deacetylase inhibitors;
  • cell cycle;
  • leukemia;
  • clinical trials