UNIT 8.2 Basic Preparative Techniques for Fluorescence In Situ Hybridization
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Wiegant, J. and Raap, A. 2001. Basic Preparative Techniques for Fluorescence In Situ Hybridization. Current Protocols in Cytometry. 00:8.2:8.2.1–8.2.23.
- Published Online: 1 MAY 2001
- Published Print: APR 1997
This unit presents protocols for preparing human metaphase chromosome slides from peripheral blood lymphocytes, isolating interphase nuclei from lymphocytes and paraffin-embedded tissues, and preparing DNA fibers. The protocols are designed so that the resulting preparations are amenable to FISH. The methods correspond to a selection of the specimens that can be analyzed with FISH techniques, and the choice of sample preparation method is highly dependent on the molecular cytogenetics question being addressed.