UNIT 8.2 Basic Preparative Techniques for Fluorescence In Situ Hybridization

  1. J. Wiegant,
  2. A.K. Raap

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0802s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Wiegant, J. and Raap, A. 2001. Basic Preparative Techniques for Fluorescence In Situ Hybridization. Current Protocols in Cytometry. 00:8.2:8.2.1–8.2.23.

Author Information

  1. Leiden University, Leiden, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997


This unit presents protocols for preparing human metaphase chromosome slides from peripheral blood lymphocytes, isolating interphase nuclei from lymphocytes and paraffin-embedded tissues, and preparing DNA fibers. The protocols are designed so that the resulting preparations are amenable to FISH. The methods correspond to a selection of the specimens that can be analyzed with FISH techniques, and the choice of sample preparation method is highly dependent on the molecular cytogenetics question being addressed.