Unit

UNIT 8.3 Probe Labeling and Fluorescence In Situ Hybridization

  1. J. Wiegant (probe labeling),
  2. A.K. Raap (FISH)

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0803s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Wiegant, J. and Raap, A. 2001. Probe Labeling and Fluorescence In Situ Hybridization. Current Protocols in Cytometry. 00:8.3:8.3.1–8.3.21.

Author Information

  1. Leiden University, Leiden, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997

Abstract

This unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post-hybridization washes. Support protocols for probe labeling cover probe purification and quality control. Keywords: molecular cytogenetics; FISH; nick translation; random priming; PCR labeling