UNIT 8.3 Probe Labeling and Fluorescence In Situ Hybridization
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Wiegant, J. and Raap, A. 2001. Probe Labeling and Fluorescence In Situ Hybridization. Current Protocols in Cytometry. 00:8.3:8.3.1–8.3.21.
- Published Online: 1 MAY 2001
- Published Print: APR 1997
This unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post-hybridization washes. Support protocols for probe labeling cover probe purification and quality control. Keywords: molecular cytogenetics; FISH; nick translation; random priming; PCR labeling