UNIT 8.4 Immunocytochemical Detection
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Wiegant, J. 2001. Immunocytochemical Detection. Current Protocols in Cytometry. 4:8.4:8.4.1–8.4.18.
- Published Online: 1 MAY 2001
- Published Print: APR 1998
This unit provides detailed descriptions for direct and indirect in situ hybridization methods. Protocols are designed for bright-field microscopy using a precipitating chromogenic substrate and for immunofluorescence detection using a hapten-labeled probe. Several alternative procedures are provided together with an excellent discussion of the strategy used for selecting a detection method.