UNIT 8.4 Immunocytochemical Detection

  1. J. Wiegant

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0804s04

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Wiegant, J. 2001. Immunocytochemical Detection. Current Protocols in Cytometry. 4:8.4:8.4.1–8.4.18.

Author Information

  1. Leiden University, Leiden, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1998


This unit provides detailed descriptions for direct and indirect in situ hybridization methods. Protocols are designed for bright-field microscopy using a precipitating chromogenic substrate and for immunofluorescence detection using a hapten-labeled probe. Several alternative procedures are provided together with an excellent discussion of the strategy used for selecting a detection method.