UNIT 8.5 Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Hopman, A. and Ramaekers, F. 2001. Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics. Current Protocols in Cytometry. 5:8.5:8.5.1–8.5.22.
- Published Online: 1 MAY 2001
- Published Print: JUL 1998
This unit describes in detail the protocols for preparation of biological specimens (fresh or fixed) for the purposes of establishing in situ hybridization procedures using probes for specific regions of the genome, allowing targeted detection of numerical and structural chromosome aberrations in the interphase nucleus. The authors present a series of comprehensive protocols from the simplest to the most complex.
Keywords: molecular cytogenetics; in situ hybridization; chromosome aberrations; interphase cytogenetics.