Unit

UNIT 8.5 Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics

  1. A.H.M. Hopman,
  2. F.C.S. Ramaekers

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0805s05

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Hopman, A. and Ramaekers, F. 2001. Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics. Current Protocols in Cytometry. 5:8.5:8.5.1–8.5.22.

Author Information

  1. University of Maastricht, Maastricht, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1998

Abstract

This unit describes in detail the protocols for preparation of biological specimens (fresh or fixed) for the purposes of establishing in situ hybridization procedures using probes for specific regions of the genome, allowing targeted detection of numerical and structural chromosome aberrations in the interphase nucleus. The authors present a series of comprehensive protocols from the simplest to the most complex.

Keywords: molecular cytogenetics; in situ hybridization; chromosome aberrations; interphase cytogenetics.