UNIT 8.6 Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Stap, J., Aten, J. A., Lillington, D., Shelling, A. and Young, B. 2001. Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics. Current Protocols in Cytometry. 5:8.6:8.6.1–8.6.23.
- Published Online: 1 MAY 2001
- Published Print: JUL 1998
The methods covered in this unit include flow cytometry of metaphase chromosomes, chromosome dissection, and the DOP-PCR amplification methods for reverse chromosome painting. Successful application in these areas requires care and attention to methodological details, and this unit is particularly comprehensive.
Keywords: molecular cytogenetics; chromosomes; flow cytometry; microdissection; metaphase; cytogenetics.