UNIT 8.6 Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics

  1. Jan Stap1,
  2. Jacob A. Aten1,
  3. D. Lillington2,
  4. A. Shelling2,
  5. B.D. Young2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0806s05

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Stap, J., Aten, J. A., Lillington, D., Shelling, A. and Young, B. 2001. Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics. Current Protocols in Cytometry. 5:8.6:8.6.1–8.6.23.

Author Information

  1. 1

    University of Amsterdam, Amsterdam, The Netherlands

  2. 2

    St. Bartholomew's Hospital School of Medicine, London, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1998


The methods covered in this unit include flow cytometry of metaphase chromosomes, chromosome dissection, and the DOP-PCR amplification methods for reverse chromosome painting. Successful application in these areas requires care and attention to methodological details, and this unit is particularly comprehensive.

Keywords: molecular cytogenetics; chromosomes; flow cytometry; microdissection; metaphase; cytogenetics.