UNIT 8.7 Combination DNA/RNA Fish and Immunophenotyping
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Dirks, R. W. 2001. Combination DNA/RNA Fish and Immunophenotyping. Current Protocols in Cytometry. 6:8.7:8.7.1–8.7.14.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
This unit presents methods for combining immunophenotyping with DNA/RNA FISH. The approach is used in so-called genotype/phenotype analysis to identify chromosomal aberrations in sub-populations of cells present in heterogenous populations. Combining RNA and DNA detection with identification of cellular proteins is quite difficult. This series of protocols is provided to enable the successful application of the combination of these techniques.