Unit

UNIT 8.10 Molecular Combing

  1. Chiara Conti,
  2. Sandrine Caburet,
  3. Catherine Schurra,
  4. Aaron Bensimon

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0810s16

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Conti, C., Caburet, S., Schurra, C. and Bensimon, A. 2001. Molecular Combing. Current Protocols in Cytometry. 16:8.10:8.10.1–8.10.23.

Author Information

  1. Institut Pasteur, Paris, France

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

Abstract

This unit describes an important advance in fiber-FISH technology called molecular combing, in which single DNA molecules are bound by one or both ends to a surface and stretched in a uniform and parallel manner by a receding meniscus. This technique is gentle on the molecules, rapid, and easy to perform. Reliable, quantitative information for genome-wide studies can be obtained without the need for other techniques and a large number of accurate measurements can be made in a single experiment. The authors provide detailed protocols for basic molecular combing, high-resolution physical mapping, and gene-dosage approaches as well as support protocols outlining surface preparation, DNA solution preparation, and probe labeling.