UNIT 8.13 Combined Immunofluorescence and FISH: New Prospects for Tumor Cell Detection/Identification

  1. Peter F. Ambros1,
  2. Gábor Méhes2

Published Online: 1 NOV 2004

DOI: 10.1002/0471142956.cy0813s26

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Ambros, P. F. and Méhes, G. 2004. Combined Immunofluorescence and FISH: New Prospects for Tumor Cell Detection/Identification. Current Protocols in Cytometry. 26:8.13:8.13.1–8.13.11.

Author Information

  1. 1

    Children's Cancer Research Institute (CCRI), St. Anna Children's Hospital, Vienna, Austria

  2. 2

    University of Pécs, Pécs, Hungary

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2003


Fluorescence-microscope based methods are presented that allow automatic selection and quantification of immunophenotyped cells and sequential FISH analysis to facilitate molecular cytogenetic analysis of single cells and very small cell populations. The protocols in this unit are particularly useful for studying the biological and genetic makeup of disseminated tumor cells or other rare cells.


  • fluorescence microscopy;
  • immunofluorescence labeling;
  • FISH;
  • cytogenetic analysis