Unit

UNIT 9.1 Overview of Functional Cell Assays

  1. Peter S. Rabinovitch1,
  2. J. Paul Robinson2

Published Online: 1 MAY 2002

DOI: 10.1002/0471142956.cy0901s21

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Rabinovitch, P. S. and Robinson, J. P. 2002. Overview of Functional Cell Assays. Current Protocols in Cytometry. 00:9.1:9.1.1–9.1.6.

Author Information

  1. 1

    University of Washington, Seattle, Washington

  2. 2

    Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: JUL 2002

Abstract

Flow cytometry can measure a variety of functional parameters of increasing interest to cell biologists and clinicians. The distinguishing feature of these assays is that the information of interest is the functional behavior of the cell itself, rather than the phenotype. This unit discusses cell viability, oxidative metabolism, membrane potential, intracellular ionized calcium, intracellular pH, intracellular organelles, and gene reporter assays.