UNIT 9.2 Assessment of Cell Viability

  1. David M. Coder

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0902s15

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Coder, D. M. 2001. Assessment of Cell Viability. Current Protocols in Cytometry. 15:9.2:9.2.1–9.2.14.

Author Information

  1. University of Washington School of Medicine, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

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Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes of the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre-fixation viability of fixed cells either before or after fixation. A dye-exclusion procedure for microscopy is also included.