Unit

UNIT 9.2 Assessment of Cell Viability

  1. Simon Johnson1,
  2. Vy Nguyen1,
  3. David Coder2

Published Online: 1 APR 2013

DOI: 10.1002/0471142956.cy0902s64

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Johnson, S., Nguyen, V. and Coder, D. 2013. Assessment of Cell Viability. Current Protocols in Cytometry. 64:9.2:9.2.1–9.2.26.

Author Information

  1. 1

    University of Washington School of Medicine, Seattle, Washington

  2. 2

    Baybioscience, Irvine, California

Publication History

  1. Published Online: 1 APR 2013
  2. Published Print: APR 2013

Abstract

Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes or the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre-fixation viability of fixed cells either before or after fixation with amine-reactive dyes suitable for a range of excitation wavelengths. Membrane-impermeable dead cell and live cell dyes as well as dye-exclusion procedures for microscopy are also included. Curr. Protoc. Cytom. 64:9.2.1-9.2.26. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • cytometry;
  • flow cytometry;
  • imaging;
  • probes;
  • toxicology;
  • assessment of cell toxicity