UNIT 9.4 Analysis of Intracellular Organelles by Flow Cytometry or Microscopy
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Poot, M. 2001. Analysis of Intracellular Organelles by Flow Cytometry or Microscopy. Current Protocols in Cytometry. 14:9.4:9.4.1–9.4.24.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
Functional analysis of cellular organelles can be accomplished by staining cells with suitable organelle-specific dyes and analyzing the fluorescence of the stained cells with a flow cytometer. With this methodology it is possible to resolve suspected heterogeneity in organelle function or content within a population of cells. Morphological information can be provided by quantitative microscopy (confocal microscope or video microscope with digital image-analysis system). The thirteen protocols cover flow cytometry and microscopy for both live and fixed cells.