Unit

UNIT 9.4 Analysis of Intracellular Organelles by Flow Cytometry or Microscopy

  1. Martin Poot

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0904s14

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Poot, M. 2001. Analysis of Intracellular Organelles by Flow Cytometry or Microscopy. Current Protocols in Cytometry. 14:9.4:9.4.1–9.4.24.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

Abstract

Functional analysis of cellular organelles can be accomplished by staining cells with suitable organelle-specific dyes and analyzing the fluorescence of the stained cells with a flow cytometer. With this methodology it is possible to resolve suspected heterogeneity in organelle function or content within a population of cells. Morphological information can be provided by quantitative microscopy (confocal microscope or video microscope with digital image-analysis system). The thirteen protocols cover flow cytometry and microscopy for both live and fixed cells.