UNIT 9.7 Oxidative Metabolism

  1. J. Paul Robinson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0907s02

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Robinson, J. P. 2001. Oxidative Metabolism. Current Protocols in Cytometry. 2:9.7:9.7.1–9.7.14.

Author Information

  1. Purdue University Cytometry Laboratories, West Lafayette, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1997


Peroxide production is detected using DCFH-DA, superoxide anion by hydroethidine; the methods can be combined for simultaneous detection. A support protocol describes calibration of the flow cytometer to permit calculation of the actual H2O2 production per cell. These protocols are applicable to many different cell types, but provide detailed information on using neutrophils, highly reactive cells that produce vast quantities of ROS.