UNIT 9.8 Measurement of Intracellular Calcium Ions by Flow Cytometry
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
June, C. H., Abe, R. and Rabinovitch, P. S. 2001. Measurement of Intracellular Calcium Ions by Flow Cytometry. Current Protocols in Cytometry. 2:9.8:9.8.1–9.8.19.
- Published Online: 1 MAY 2001
- Published Print: OCT 1997
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Using flow cytometry, single-cell measurements of calcium can be made on isolated populations identified by one or more phenotypic characteristics. This unit describes the preparation of cells, including labeling with antibodies and with calcium probes, and discusses the principles of data analysis and interpretation.