UNIT 9.8 Measurement of Intracellular Calcium Ions by Flow Cytometry

  1. Carl H. June1,
  2. Ryo Abe1,
  3. Peter S. Rabinovitch2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0908s02

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

June, C. H., Abe, R. and Rabinovitch, P. S. 2001. Measurement of Intracellular Calcium Ions by Flow Cytometry. Current Protocols in Cytometry. 2:9.8:9.8.1–9.8.19.

Author Information

  1. 1

    Naval Medical Research Institute, Bethesda, Maryland

  2. 2

    University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1997

This is not the most recent version of the article. View current version (1 APR 2015)


Using flow cytometry, single-cell measurements of calcium can be made on isolated populations identified by one or more phenotypic characteristics. This unit describes the preparation of cells, including labeling with antibodies and with calcium probes, and discusses the principles of data analysis and interpretation.