UNIT 9.9 Intracellular Cytokines

  1. George F. Babcock

Published Online: 1 MAY 2004

DOI: 10.1002/0471142956.cy0909s28

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Babcock, G. F. 2004. Intracellular Cytokines. Current Protocols in Cytometry. 28:9.9:9.9.1–9.9.11.

Author Information

  1. University of Cincinnati College of Medicine and Shriners Burns Institute, Cincinnati, Ohio

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: APR 2004


The principal technique described in this unit was developed for detecting cytokines produced by T cells, specifically interferon gamma (IFN-γ) and interleukin 4 (IL-4). In addition to providing information about which cytokines are being produced, it also helps to phenotypically identify the specific cells producing them. For example, CD4+ or CD8+ cells can be subdivided into TH1/TH2 helper cells or TC1/TC2 suppressor cells, respectively. This procedure can be useful in examining the response of cells to a variety of agonists, the immune function in various disease states, and the level of lymphocyte activation or suppression. In addition, it can be used to detect a variety of cytokines in many cell types, although the methodology must be carefully evaluated for each cytokine or cell type of interest. Additional methods are included for the stimulation of T cells with allogeneic cells and for the performance of controls for labeling specificity.


  • T cells;
  • cytokines;
  • immune function;
  • lymphocyte activation;
  • interferon gamma;
  • interleukin 4