UNIT 9.12 Reporters of Gene Expression: Autofluorescent Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Rasko, J. E. 2001. Reporters of Gene Expression: Autofluorescent Proteins. Current Protocols in Cytometry. 7:9.12:9.12.1–9.12.16.
- Published Online: 1 MAY 2001
- Published Print: JAN 1999
This unit on GFP provides clear, detailed, easy-to-follow directions for successful transduction of GFP into appropriate cell lines, as well as carefully detailed strategy and trouble-shooting sections. Instructions for sorting and purification of sucessfully transduced cells and for analysis and re-analysis of cells together with sample data are all included. The discussion provides data on multiple GFP mutants and lists relevant sources and suggested reading material.