Unit

UNIT 9.12 Reporters of Gene Expression: Autofluorescent Proteins

  1. John E.J. Rasko

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0912s07

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Rasko, J. E. 2001. Reporters of Gene Expression: Autofluorescent Proteins. Current Protocols in Cytometry. 7:9.12:9.12.1–9.12.16.

Author Information

  1. Centenary Institute of Cancer Medicine and Cell Biology, Sydney, Australia

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1999

Abstract

This unit on GFP provides clear, detailed, easy-to-follow directions for successful transduction of GFP into appropriate cell lines, as well as carefully detailed strategy and trouble-shooting sections. Instructions for sorting and purification of sucessfully transduced cells and for analysis and re-analysis of cells together with sample data are all included. The discussion provides data on multiple GFP mutants and lists relevant sources and suggested reading material.