UNIT 9.13 In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Bowden, E. T., Mueller, S. and Coopman, P. J. 2001. In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix. Current Protocols in Cytometry. 12:9.13:9.13.1–9.13.8.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
Degradation of the extracellular matrix is an essential component of phagocytosis by tumor cells and can be correlated with their invasive capacity. This unit presents flow cytometry based assays for rapid quantitative assessment of both proteolysis and internalization or internalization alone. These assays provide high-throughput, low-cost methods to compare cell lines and test the efficacy of treatments designed to stimulate or inhibit invasion.