Unit

UNIT 9.13 In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix

  1. Emma T. Bowden1,
  2. Susette Mueller1,
  3. Peter J. Coopman2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0913s12

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Bowden, E. T., Mueller, S. and Coopman, P. J. 2001. In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix. Current Protocols in Cytometry. 12:9.13:9.13.1–9.13.8.

Author Information

  1. 1

    Georgetown University Medical Center, Washington, D.C.

  2. 2

    Montpellier University II, Montpellier, France

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000

Abstract

Degradation of the extracellular matrix is an essential component of phagocytosis by tumor cells and can be correlated with their invasive capacity. This unit presents flow cytometry based assays for rapid quantitative assessment of both proteolysis and internalization or internalization alone. These assays provide high-throughput, low-cost methods to compare cell lines and test the efficacy of treatments designed to stimulate or inhibit invasion.