Unit

UNIT 9.14 Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1

  1. Andrea Cossarizza,
  2. Stefano Salvioli

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0914s13

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Cossarizza, A. and Salvioli, S. 2001. Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1. Current Protocols in Cytometry. 13:9.14:9.14.1–9.14.7.

Author Information

  1. University of Modena, Modena, Italy

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

Changes in membrane potential have long been known to represent early activation events. This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC-1 probe for measuring mitochondrial membrane potential by flow cytometry. A valuable component of this measurement system is the possibility of making quantitative measurements of changes in membrane potential.