UNIT 9.14 Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Cossarizza, A. and Salvioli, S. 2001. Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1. Current Protocols in Cytometry. 13:9.14:9.14.1–9.14.7.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
Changes in membrane potential have long been known to represent early activation events. This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC-1 probe for measuring mitochondrial membrane potential by flow cytometry. A valuable component of this measurement system is the possibility of making quantitative measurements of changes in membrane potential.