Unit

UNIT 9.15 Multiparameter Analysis of Physiological Changes in Apoptosis

  1. Martin Poot

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0915s14

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Poot, M. 2001. Multiparameter Analysis of Physiological Changes in Apoptosis. Current Protocols in Cytometry. 14:9.15:9.15.1–9.15.7.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

Abstract

Changes in mitochondrial parameters leading to cells with compromised mitochondrial function are a hallmark of apoptosis. These changes can be detected and the number of compromised cells quantitated by multicolor flow cytometry utilizing fluorescent dyes that monitor specific features of this organelle. In this way one can trace physiological changes during apoptosis and investigate whether or not certain changes occur simultaneously in the same cell. This unit presents protocols for combined NADH levels and mitochondrial membrane potential, cell cycle stage specific apoptosis, and cellular thiol and mitochondrial cardiolipin levels.