Unit

UNIT 9.19 Assessment of Phagocyte Functions by Flow Cytometry

  1. Carl-Fredrik Bassøe

Published Online: 1 AUG 2002

DOI: 10.1002/0471142956.cy0919s21

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Bassøe, C.-F. 2002. Assessment of Phagocyte Functions by Flow Cytometry. Current Protocols in Cytometry. 21:9.19:9.19.1–9.19.22.

Author Information

  1. Haukeland University Hospital, Bergen, Norway

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUL 2002

Abstract

Phagocytes neutrophils, monocytes, and macrophages are crucial in the defense against infection. Their functions include phagocytosis, intra- and extracellular digestion of targets, oxidative burst, and chemotaxis. This extensive, detailed unit outlines a four-part procedure for in-depth investigation of these four functions that reflect the attacking and processing of pathogenic microorganisms. Written by the original instigator of flow-based assays in this area, this unit defines an area that is as old as flow itself. Numerous support protocols provide preparative procedures for bacterial targets, opsonins, and antigen-coated beads.