Unit

UNIT 9.21 Flow Cytometric Analysis of Cytokine Responses in Stimulated Whole Blood: Simultaneous Quantitation of TNF-α-Secreting Cells and Soluble Cytokines

  1. Arancha Rodríguez-Caballero,
  2. Andrés García-Montero,
  3. Clara Bueno,
  4. Alberto Orfao

Published Online: 1 AUG 2003

DOI: 10.1002/0471142956.cy0921s25

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Rodríguez-Caballero, A., García-Montero, A., Bueno, C. and Orfao, A. 2003. Flow Cytometric Analysis of Cytokine Responses in Stimulated Whole Blood: Simultaneous Quantitation of TNF-α-Secreting Cells and Soluble Cytokines. Current Protocols in Cytometry. 25:9.21:9.21.1–9.21.21.

Author Information

  1. Universidad de Salamanca, Salamanca, Spain

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUL 2003

Abstract

This unit describes technical protocols aimed at the ex vivo or in vitro evaluation of the functional status of the immune system through the simultaneous identification and enumeration of cytokine-secreting cells and quantitation of the soluble cytokines produced by these cells.

Keywords:

  • Cytokines;
  • TNF-α;
  • immune system;
  • cell activation;
  • immunoassay