UNIT 9.29 Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods
Published Online: 1 JAN 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Zuba-Surma, E. K. and Ratajczak, M. Z. 2010. Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods. Current Protocols in Cytometry. 51:9.29:9.29.1–9.29.15.
- Published Online: 1 JAN 2010
- Published Print: JAN 2010
The protocols presented here describe the procedures employed to identify and isolate very small embryonic-like stem cells (VSELs) using flow cytometric technologies including fluorescence-activated cell sorting (FACS). We describe the recommended steps in detail for their successful identification and isolation from adult tissues. These protocols were initially established to isolate such cells from murine bone marrow (BM) and human cord blood (CB) and may also be employed to isolate these primitive cells from other adult organs and embryonic tissues. Here, we focus on some critical parameters/key points required for the successful identification and purification of these rare cells by employing classical flow cytometry. In the last part of this unit, we also discuss a novel flow cytometric tool, ImageStream, an imaging flow cytometer, which allows better identification and morphological analysis of sorted cells. Curr. Protoc. Cytom. 51:9.29.1-9.29.15. © 2010 by John Wiley & Sons, Inc.
- flow cytometry;
- pluripotent stem cells