Unit

UNIT 9.29 Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods

  1. Ewa K. Zuba-Surma,
  2. Mariusz Z. Ratajczak

Published Online: 1 JAN 2010

DOI: 10.1002/0471142956.cy0929s51

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Zuba-Surma, E. K. and Ratajczak, M. Z. 2010. Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods. Current Protocols in Cytometry. 51:9.29:9.29.1–9.29.15.

Author Information

  1. Stem Cell Biology Institute, University of Louisville, Louisville, Kentucky

Publication History

  1. Published Online: 1 JAN 2010
  2. Published Print: JAN 2010

Abstract

The protocols presented here describe the procedures employed to identify and isolate very small embryonic-like stem cells (VSELs) using flow cytometric technologies including fluorescence-activated cell sorting (FACS). We describe the recommended steps in detail for their successful identification and isolation from adult tissues. These protocols were initially established to isolate such cells from murine bone marrow (BM) and human cord blood (CB) and may also be employed to isolate these primitive cells from other adult organs and embryonic tissues. Here, we focus on some critical parameters/key points required for the successful identification and purification of these rare cells by employing classical flow cytometry. In the last part of this unit, we also discuss a novel flow cytometric tool, ImageStream, an imaging flow cytometer, which allows better identification and morphological analysis of sorted cells. Curr. Protoc. Cytom. 51:9.29.1-9.29.15. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • VSELs;
  • flow cytometry;
  • ImageStream;
  • Oct-4;
  • pluripotent stem cells