Unit

UNIT 9.31 Measurement of Phagocytosis and of the Phagosomal Environment in Polymorphonuclear Phagocytes by Flow Cytometry

  1. Elizabeth R. Simons

Published Online: 1 JAN 2010

DOI: 10.1002/0471142956.cy0931s51

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Simons, E. R. 2010. Measurement of Phagocytosis and of the Phagosomal Environment in Polymorphonuclear Phagocytes by Flow Cytometry. Current Protocols in Cytometry. 51:9.31:9.31.1–9.31.10.

Author Information

  1. Boston University School of Medicine, Boston, Massachusetts

Publication History

  1. Published Online: 1 JAN 2010
  2. Published Print: JAN 2010

Abstract

Phagocytes are the most important early components of the immune response, programmed to recognize, engulf, and destroy immune complexes (formed when antibodies recognize their specific antigens), foreign particles, bacteria, mycobacteria, apoptotic cells, etc. Neutrophils, monocytes, macrophages, and dendritic cells all participate in this process. Flow cytometry permits observation of phagocytes that have responded and, with the appropriate fluorescent probes, of the environment in the phagosome that has enclosed the foreign matter. This unit gives the background and the protocols for performing such studies. Curr. Protoc. Cytom. 51:9.31.1-9.31.10. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • phagocytosis;
  • phagosome;
  • phagocytes;
  • flow cytometry