Unit

UNIT 9.32 Yeast Cell Cycle Analysis: Combining DNA Staining with Cell and Nuclear Morphology

  1. Meredith E.K. Calvert1,
  2. Joanne Lannigan2

Published Online: 1 APR 2010

DOI: 10.1002/0471142956.cy0932s52

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Calvert, M. E. and Lannigan, J. 2010. Yeast Cell Cycle Analysis: Combining DNA Staining with Cell and Nuclear Morphology. Current Protocols in Cytometry. 53:9.32:9.32.1–9.32.16.

Author Information

  1. 1

    Temasek Life Sciences Laboratory, National University of Singapore, Singapore

  2. 2

    University of Virginia, Charlottesville, Virginia

Publication History

  1. Published Online: 1 APR 2010
  2. Published Print: APR 2010

Abstract

In studies of eukaryotic cell cycle regulation, the budding yeast Saccharoymyces cerevisiae offers many advantages as a model system. Due to its simple growth requirements and genetic tractability, this organism is a powerful tool for investigating the molecular regulation of cell cycle control. One earlier disadvantage to performing cell cycle analyses in yeast was that existing methods were restricted to either visual analysis or flow cytometry, both of which present limitations in the scope and accuracy of the data obtained. This unit demonstrates the combined use of DNA content measurements and bright-field image analysis using multispectral imaging flow cytometry (MIFC) to provide a more precise quantitation of yeast cell cycle distribution. The advantage of this method is the ability to analyze large numbers of cells using multiple cell cycle indicators in a relatively short amount of time. Curr. Protoc. Cytom. 52:9.32.1-9.32.16. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • budding yeast;
  • cell cycle;
  • MIFC;
  • multispectral imaging flow cytometry;
  • ImageStream;
  • morphometrics;
  • morphology;
  • image analysis;
  • DNA content