Unit

UNIT 9.37 Assessing Mitochondrial Redox Status by Flow Cytometric Methods: Vascular Response to Fluid Shear Stress

  1. Rongsong Li,
  2. Nelson Jen,
  3. Fei Yu,
  4. Tzung K. Hsiai

Published Online: 1 OCT 2011

DOI: 10.1002/0471142956.cy0937s58

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Li, R., Jen, N., Yu, F. and Hsiai, T. K. 2011. Assessing Mitochondrial Redox Status by Flow Cytometric Methods: Vascular Response to Fluid Shear Stress. Current Protocols in Cytometry. 58:9.37:9.37.1–9.37.14.

Author Information

  1. Cardiovascular Engineering Research, Department of Biomedical Engineering and Cardiology, School of Medicine and School of Engineering, University of Southern California, Los Angeles, California

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011

Abstract

Mitochondria are an important source of superoxide production contributing to physiological and pathological responses, including vascular oxidative stress that is relevant to cardiovascular diseases. Vascular oxidative stress is intimately linked with pro-inflammatory states and atherosclerosis. Oxidized low-density lipoprotein (OxLDL) modulates intracellular redox status and induces apoptosis in endothelial cells. Hemodynamic, specifically, fluid shear stress imparts both biomechanical and metabolic effects on vasculature. Mitochondria are an important source of superoxide production contributing to vascular oxidative stress with relevance to cardiovascular diseases. We hereby present biophysical and biochemical approaches, including fluorescence-activated cell sorting, to assess the dynamics of vascular redox status. Curr. Protoc. Cytom. 58:9.37.1-9.37.14. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • flow cytometry;
  • oxidative stress;
  • shear stress;
  • mitochondrial redox status