UNIT 9.40 Flow Cytometry-Based Quantification of Cell Proliferation in the Mixed Cell Co-Culture

  1. Bogdan I. Gerashchenko1,
  2. Roger W. Howell2

Published Online: 1 JAN 2013

DOI: 10.1002/0471142956.cy0940s63

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Gerashchenko, B. I. and Howell, R. W. 2013. Flow Cytometry-Based Quantification of Cell Proliferation in the Mixed Cell Co-Culture. Current Protocols in Cytometry. 63:9.40:9.40.1–9.40.10.

Author Information

  1. 1

    Department of Radiobiology and Ecology, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine

  2. 2

    Department of Radiology, UMDNJ−New Jersey Medical School Cancer Center, Newark, New Jersey

Publication History

  1. Published Online: 1 JAN 2013
  2. Published Print: JAN 2013


In cell communities, among the crucial signals that govern cell function are those generated locally by surrounding cells. A co-culture of mixed homotypic or heterotypic cells, which is often used in various fields of experimental biology and medicine, can be applied for elucidation of the role of cell proximity in modulating proliferative responses. Quick and reliable quantification of the changes in proliferation of each of the mixed cell populations as a result of their co-culture is of importance. For this purpose, flow cytometry together with fluorescent tracers that do not affect cell proliferation can be used. Curr. Protoc. Cytom. 63:9.40.1-9.40.10. © 2013 by John Wiley & Sons, Inc.


  • cell proliferation;
  • cell proximity;
  • mixed co-culture;
  • fluorescent cell tracers;
  • flow cytometry;
  • proliferation ratio