Unit

UNIT 10.9 Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry

  1. Laura Mascio

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy1009s03

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Mascio, L. 2001. Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry. Current Protocols in Cytometry. 3:10.9:10.9.1–10.9.11.

Author Information

  1. Lawrence Livermore National Laboratory, Livermore, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1998

Abstract

Because of time considerations in performing image analysis for detection of very small amounts of fluorescence probe, there are advantages in using automated detection systems. This unit provides considerable detail on several algorithms used for location of hybridization domains that reduce the tedium and improve objectivity and repeatability. Not only are these algorithms described and discussed in detail, but sources for the programs are also provided. Here is a carefully crafted textual and graphical description of how to go about learning the art of automated image analysis.