UNIT 10.9 Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Mascio, L. 2001. Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry. Current Protocols in Cytometry. 3:10.9:10.9.1–10.9.11.
- Published Online: 1 MAY 2001
- Published Print: JAN 1998
Because of time considerations in performing image analysis for detection of very small amounts of fluorescence probe, there are advantages in using automated detection systems. This unit provides considerable detail on several algorithms used for location of hybridization domains that reduce the tedium and improve objectivity and repeatability. Not only are these algorithms described and discussed in detail, but sources for the programs are also provided. Here is a carefully crafted textual and graphical description of how to go about learning the art of automated image analysis.