Unit

UNIT 10.17 Web-Based Analysis and Publication of Flow Cytometry Experiments

  1. Nikesh Kotecha1,2,3,
  2. Peter O. Krutzik1,2,
  3. Jonathan M. Irish1

Published Online: 1 JUL 2010

DOI: 10.1002/0471142956.cy1017s53

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Kotecha, N., Krutzik, P. O. and Irish, J. M. 2010. Web-Based Analysis and Publication of Flow Cytometry Experiments. Current Protocols in Cytometry. 53:10.17:10.17.1–10.17.24.

Author Information

  1. 1

    Stanford University School of Medicine, Stanford, California

  2. 2

    Stanford University Department of Microbiology and Immunology, Stanford, California

  3. 3

    Stanford University Biomedical Informatics Program, Stanford, California

Publication History

  1. Published Online: 1 JUL 2010
  2. Published Print: JUL 2010

Abstract

Cytobank is a Web-based application for storage, analysis, and sharing of flow cytometry experiments. Researchers use a Web browser to log in and use a wide range of tools developed for basic and advanced flow cytometry. In addition to providing access to standard cytometry tools from any computer, Cytobank creates a platform and community for developing new analysis and publication tools. Figure layouts created on Cytobank are designed to allow transparent access to the underlying experiment annotation and data processing steps. Since all flow cytometry files and analysis data are stored on a central server, experiments and figures can be viewed or edited by anyone with the proper permission, from any computer with Internet access. Once a primary researcher has performed the initial analysis of the data, collaborators can engage in experiment analysis and make their own figure layouts using the gated, compensated experiment files. Cytobank is available to the scientific community at http://www.cytobank.org. Curr. Protoc. Cytom. 53:10.17.1-10.17.24. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • Cytobank;
  • database;
  • storage platform;
  • analysis;
  • flow cytometry