Unit

UNIT 11.2 Flow Cytometry and Environmental Microbiology

  1. Jonathan Porter

Published Online: 1 FEB 2004

DOI: 10.1002/0471142956.cy1102s27

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Porter, J. 2004. Flow Cytometry and Environmental Microbiology. Current Protocols in Cytometry. 27:11.2:11.2.1–11.2.13.

Author Information

  1. University of Exeter, Exeter, United Kingdom

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: JAN 2004

Abstract

This survey unit discusses many of the issues involved for flow cytometry in the field of microbiology, particularly the preparative procedures, which are far more stringent than many other procedures using larger cells. For instance, it is often necessary to filter laboratory agents multiple times to obtain the true particle-free solutions needed for flow cytometry of microbes. It is difficult enough to recognize bacteria in cell extracts from soil, sediment, or sludge given the background of same-size particles. This unit provides an excellent overview of a potentially large application area in flow cytometry and is written by one of the most respected scientists in the field.

Keywords:

  • flow cytometry;
  • cell sorting;
  • environmental microbiology;
  • viability;
  • biomass