Unit

UNIT 11.5 Detection of Borreliacidal Antibodies by Flow Cytometry

  1. Steven M. Callister1,
  2. Dean A. Jobe1,
  3. Ronald F. Schell2

Published Online: 1 NOV 2004

DOI: 10.1002/0471142956.cy1105s26

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Callister, S. M., Jobe, D. A. and Schell, R. F. 2004. Detection of Borreliacidal Antibodies by Flow Cytometry. Current Protocols in Cytometry. 26:11.5:11.5.1–11.5.12.

Author Information

  1. 1

    Gundersen Lutheran Medical Center and Microbiology Research Laboratory and Gundersen Lutheran Medical Foundation, La Crosse, Wisconsin

  2. 2

    Wisconsin State Laboratory of Hygiene and Department of Medical Microbiology and Immunology, Madison, Wisconsin

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2003

Abstract

Lyme disease is a multisystem disorder that usually begins with a skin lesion called erythema migrans and with constitutional symptoms. If the disease is left untreated or treated inappropriately, dissemination of the organism can lead to more severe sequelae, including nervous system disorders or arthritis. Vaccinations with B. burgdorferi or several individual B. burgdorferi proteins induce borreliacidal antibodies that provide protection against infection by inducing a complement cascade that kills the spirochetes without the necessity of scavenging by phagocytic cells. Detection of borreliacidal antibodies is therefore useful for serodiagnosing Lyme disease and monitoring immune status after vaccination. This unit provides a technique for detecting anti-B. burgdorferi antibodies, as well as for preparing and determining the quality of Barbour-Stoenner-Kelly (BSK medium) and complement. In addition, methods are provided for preparation of a B. burgdorferi stock and Mueller-Hinton agar containing Bacillus subtilis spores.

Keywords:

  • Borrelia burgdorferi;
  • Lyme disease;
  • borreliacidal antibodies;
  • BSK medium;
  • Mueller-Hinton agar;
  • Bacillus subtilis
  • Borrelia burgdorferi;
  • Lyme disease;
  • borreliacidal antibodies