UNIT 11.8 Antibiotic Susceptibility Testing by Flow Cytometry

  1. David J. Mason1,
  2. Fiona C. Mortimer2,
  3. Vanya A. Gant3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy1108s08

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Mason, D. J., Mortimer, F. C. and Gant, V. A. 2001. Antibiotic Susceptibility Testing by Flow Cytometry. Current Protocols in Cytometry. 8:11.8:11.8.1–11.8.9.

Author Information

  1. 1

    University of Warwick, Coventry, United Kingdom

  2. 2

    Formerly of King's College London, London, United Kingdom

  3. 3

    University College Hospital and Trust, London, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1999


Flow cytometry can provide a rapid indication of antibiotic susceptibility. This unit describes a number of fluorescent dyes that allow the cytometer to distinguish the heterogeneic nature of populations exposed to antibiotics. No single method can be used for multiple organisms, since it is the interference with the organisms growth and structural integrity that signals the impact of the antibiotic so rapidly. This unit discusses membrane structural changes as well as alterations in nucleic acid and the probes that can be successfully used with flow cytometry.