UNIT 11.8 Antibiotic Susceptibility Testing by Flow Cytometry
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Mason, D. J., Mortimer, F. C. and Gant, V. A. 2001. Antibiotic Susceptibility Testing by Flow Cytometry. Current Protocols in Cytometry. 8:11.8:11.8.1–11.8.9.
- Published Online: 1 MAY 2001
- Published Print: APR 1999
Flow cytometry can provide a rapid indication of antibiotic susceptibility. This unit describes a number of fluorescent dyes that allow the cytometer to distinguish the heterogeneic nature of populations exposed to antibiotics. No single method can be used for multiple organisms, since it is the interference with the organisms growth and structural integrity that signals the impact of the antibiotic so rapidly. This unit discusses membrane structural changes as well as alterations in nucleic acid and the probes that can be successfully used with flow cytometry.