Unit

UNIT 11.9 Determination of Bacterial Biomass from Flow Cytometric Measurements of Forward Light Scatter Intensity

  1. B.R. Robertson,
  2. D.K. Button

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy1109s09

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Robertson, B. and Button, D. 2001. Determination of Bacterial Biomass from Flow Cytometric Measurements of Forward Light Scatter Intensity. Current Protocols in Cytometry. 9:11.9:11.9.1–11.9.10.

Author Information

  1. University of Alaska Fairbanks, Fairbanks, Alaska

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1999

Abstract

This unit presents methods for measuring biomass of bacteria by flow cytometry. Such measurements are an integral part of determining growth rates and kinetics. This unit discusses calibration and standardization methods for bacterial studies using flow cytometry and provides a detailed discussion of the components involved. The serious microbiologist will appreciate the advantages flow cytometry brings to many aspects of microbial study, and this unit is no exception.