Unit

UNIT 11.12 DNA/RNA Analysis of Phytoplankton by Flow Cytometry

  1. Dominique Marie,
  2. Nathalie Simon,
  3. Laure Guillou,
  4. Frédéric Partensky,
  5. Daniel Vaulot

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy1112s11

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Marie, D., Simon, N., Guillou, L., Partensky, F. and Vaulot, D. 2001. DNA/RNA Analysis of Phytoplankton by Flow Cytometry. Current Protocols in Cytometry. 11:11.12:11.12.1–11.12.14.

Author Information

  1. INSU et Université Pierre et Marie Curie, Roscoff, France

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2000

Abstract

The past 10 years or so have seen the combination of molecular and biochemical techniques within the confines of cytometry. The use of flow cytometry in microbiology is finally coming of age. This unit carefully defines the criteria for evaluation of DNA and RNA in phytoplankton. Of course not everyone works with phytoplankton, but the methods outlined are very appropriately representative for other organisms. In addition, the unit discusses the methods for evaluating cell cycle and discriminating specific taxa using fluorescent oligonucleotide probes targeted to 18S rRNA.