UNIT 11.14 Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Nuñez, R., Kamau, S. and Grimm, F. 2001. Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes. Current Protocols in Cytometry. 15:11.14.1–11.14.9.
- Published Online: 1 MAY 2001
- Published Print: JAN 2001
Flow cytometry can be a valuable tool for parasitology in studies of parasite-drug interaction and cellular toxicity. This unit presents protocols for assessment of Leishmania promastigote proliferation, viability, and cellular protein content. The cytometric approach permits one to detect, differentiate, and quantify cellular changes in these parasites resulting from drug treatment, in this case allopurinol, as well as demonstrate differences in drug susceptibility between promastigote forms. Rapid and relatively simple flow cytometry replaces incorporation of [3H]-thymidine, hitherto the most common method for determining cell division.