Unit

UNIT 11.14 Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes

  1. Rafael Nuñez,
  2. Sarah Kamau,
  3. Felix Grimm

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy1114s15

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Nuñez, R., Kamau, S. and Grimm, F. 2001. Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes. Current Protocols in Cytometry. 15:11.14.1–11.14.9.

Author Information

  1. University of Zürich, Zürich, Switzerland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

Abstract

Flow cytometry can be a valuable tool for parasitology in studies of parasite-drug interaction and cellular toxicity. This unit presents protocols for assessment of Leishmania promastigote proliferation, viability, and cellular protein content. The cytometric approach permits one to detect, differentiate, and quantify cellular changes in these parasites resulting from drug treatment, in this case allopurinol, as well as demonstrate differences in drug susceptibility between promastigote forms. Rapid and relatively simple flow cytometry replaces incorporation of [3H]-thymidine, hitherto the most common method for determining cell division.