Unit

UNIT 11.19 Life Cycle Analysis of Unicellular Algae

  1. Bogdan I. Gerashchenko1,
  2. Toshiyuki Takahashi2,
  3. Toshikazu Kosaka3,
  4. Hiroshi Hosoya3

Published Online: 1 APR 2010

DOI: 10.1002/0471142956.cy1119s52

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Gerashchenko, B. I., Takahashi, T., Kosaka, T. and Hosoya, H. 2010. Life Cycle Analysis of Unicellular Algae. Current Protocols in Cytometry. 52:11.19:11.19.1–11.19.6.

Author Information

  1. 1

    R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine

  2. 2

    Miyakonojo National College of Technology, Miyakonojo, Japan

  3. 3

    Hiroshima University, Hiroshima, Japan

Publication History

  1. Published Online: 1 APR 2010
  2. Published Print: APR 2010

Abstract

Unicellular green alga is a very convenient object for flow cytometric characterization. Flow cytometry has been proposed as a quick and reliable tool for studying life cycle and growth of unicellular algae. Cell size of vegetating algae can be monitored in association with their DNA and endogenous chlorophyll content. Cells of interest (e.g., group of cells of a certain stage of the life cycle) in an asynchronously proliferating cell population can be sorted out for further microscopical or molecular biology studies. This methodological approach can be helpful for researchers who are interested in algal proliferation. Curr. Protoc. Cytom. 52:11.19.1-11.19.6. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • unicellular algae;
  • Chlorella;
  • light scatter;
  • DNA content;
  • chlorophyll content;
  • life cycle;
  • flow cytometry