UNIT 12.2 Basics of Digital Microscopy

  1. Tytus Bernas

Published Online: 1 FEB 2005

DOI: 10.1002/0471142956.cy1202s31

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Bernas, T. 2005. Basics of Digital Microscopy. Current Protocols in Cytometry. 31:12.2:12.2.1–12.2.14.

Author Information

  1. Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 FEB 2005
  2. Published Print: JAN 2005


Digital microscopy combines the equipment of classic light microscopy with a computerized imaging system, i.e., a digital camera. This technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, and characteristics of image file formats. Knowledge with regard to these limitations serves, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging.


  • Resolution;
  • sampling;
  • quantization;
  • signal-to-noise ratio;
  • image file format;
  • compression