UNIT 12.2 Basics of Digital Microscopy
Published Online: 1 FEB 2005
Copyright © 2005 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Bernas, T. 2005. Basics of Digital Microscopy. Current Protocols in Cytometry. 31:12.2:12.2.1–12.2.14.
- Published Online: 1 FEB 2005
- Published Print: JAN 2005
Digital microscopy combines the equipment of classic light microscopy with a computerized imaging system, i.e., a digital camera. This technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, and characteristics of image file formats. Knowledge with regard to these limitations serves, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging.
- signal-to-noise ratio;
- image file format;