Unit

UNIT 12.5 Three-Dimensional Visualization of Blood and Lymphatic Vasculature in Tissue Whole Mounts Using Confocal Microscopy

  1. Alexandra Eichten,
  2. H.-C. Jennifer Shen,
  3. Lisa M. Coussens

Published Online: 1 MAY 2005

DOI: 10.1002/0471142956.cy1205s32

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Eichten, A., Jennifer Shen, H.-C. and Coussens, L. M. 2005. Three-Dimensional Visualization of Blood and Lymphatic Vasculature in Tissue Whole Mounts Using Confocal Microscopy. Current Protocols in Cytometry. 32:12.5:12.5.1–12.5.11.

Author Information

  1. University of California at San Francisco, San Francisco

Publication History

  1. Published Online: 1 MAY 2005
  2. Published Print: APR 2005

Abstract

Two vascular systems, cardiovascular and lymphatic, maintain appropriate interstitial and intravascular fluid volume in the body. Each is endowed with innate physiologic response capabilities activated upon tissue or organ “damage.” Chronic activation following pathologic assault, however, can contribute to pathogenesis. Three-dimensional visualization of vasculature in whole tissues using confocal microscopy is a valuable tool for examining cellular and architectural changes accompanying altered vascular function. The relative affinities of plant lectins for carbohydrate moieties present on luminal surfaces of endothelial cells can be used to characterize endothelium in distinctive physiologic and pathologic states. Perivascular cells that wrap around blood endothelial cells can be visualized using antibodies immunoreactive with α-smooth muscle actin. Similarly, lymphatic endothelial cells can be detected by antibodies immunoreactive to the hyaluronan receptor LYVE-1. Together, these approaches allow functional and morphological analysis of blood vasculature distinct from endothelial cells within the lymphatic vascular network and surrounding support cells.

Keywords:

  • blood vasculature;
  • vascular leakage;
  • lymphatics;
  • whole mounts;
  • fluorescence;
  • confocal microscopy