UNIT 12.15 Cryosectioning

  1. Simon Watkins

Published Online: 1 APR 2009

DOI: 10.1002/0471142956.cy1215s48

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Watkins, S. 2009. Cryosectioning. Current Protocols in Cytometry. 48:12.15:12.15.1–12.15.7.

Author Information

  1. University of Pittsburgh Medical School, Pittsburgh, Pennsylvania

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009


This unit describes sample preparation and sectioning methods for frozen tissue. Sections of this type are used in a variety of light microscopic procedures including in situ hybridization, immunohistochemistry, and enzyme histochemistry. Curr. Protocol. Cytom. 48:12.15.1-12.15.7. © 2009 by John Wiley & Sons, Inc.


  • fixation;
  • frozen;
  • cryosections;
  • immunohistochemisty;
  • antibody