UNIT 12.16 Immunohistochemistry
Published Online: 1 APR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Watkins, S. 2009. Immunohistochemistry. Current Protocols in Cytometry. 48:12.16:12.16.1–12.16.10.
- Published Online: 1 APR 2009
- Published Print: APR 2009
Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. Curr. Protocol. Cytom. 48:12.16.1-12.16.10. © 2009 by John Wiley & Sons, Inc.